ÐÂÎÅ×ÊѶ
HPLC ²¨³¤Çл»·¨Í¬Ê±²â¶¨Òæ°²ÄþÍèÖÐ 7 ÖÖ»îÐԳɷÖ
·¢²¼Ê±¼ä£º
2022-05-12 14:52
HPLC ²¨³¤Çл»·¨Í¬Ê±²â¶¨Òæ°²ÄþÍèÖÐ 7 ÖÖ»îÐԳɷÖ
ÕÅÎÄ×Ú£¬£¬£¬ £¬£¬ÕÅ è±
ºÓÄÏÖÐÒ½Ò©´óѧ£¬£¬£¬ £¬£¬ºÓÄÏ Ö£ÖÝ 450046
Õª Òª£ºÄ¿µÄ ½¨Á¢ HPLC ²¨³¤Çл»·¨Í¬Ê±²â¶¨Òæ°²ÄþÍèÖÐ 7 ÖÖ»îÐԳɷֵķ½·¨¡£¡£¡£¡£¡£·½·¨ ²ÉÓà HPLC ·¨£¬£¬£¬ £¬£¬Í¬Ê±²â¶¨Òæ°²ÄþÍèÖÐÈýÆßÔíÜÕR1¡¢Îåζ×Ó´¼¼×¡¢µ¤·ÓËáB¡¢Îåζ×Óõ¥¼×¡¢È˲ÎÔíÜÕ Rg1¡¢È˲ÎÔíÜÕ Rb1¡¢µ¤²Îͪ IIA µÄº¬Á¿£¬£¬£¬ £¬£¬²ÉÓÃShim-pack GIST-C18 É«Æ×Öù(250 mm×4.6 mm£¬£¬£¬ £¬£¬5 μm)£¬£¬£¬ £¬£¬Á÷¶¯ÏàΪÒÒëæ-0.1%Á×ËáË®ÈÜÒº£¬£¬£¬ £¬£¬ÌݶÈÏ´ÍÑ£º0¡«5 min£¬£¬£¬ £¬£¬5%ÒÒëæ;5¡«12 min£¬£¬£¬ £¬£¬ 5%¡«12%ÒÒëæ;12¡«17 min£¬£¬£¬ £¬£¬12%¡«20%ÒÒëæ;17¡«30 min£¬£¬£¬ £¬£¬20%ÒÒëæ;30¡«36 min£¬£¬£¬ £¬£¬20%¡«50%ÒÒëæ;36¡«45 min£¬£¬£¬ £¬£¬50%¡«80%ÒÒëæ;45¡«70 min£¬£¬£¬ £¬£¬80%ÒÒëæ;¼ì²â²¨³¤ 203 nm(ÈýÆßÔíÜÕR1¡¢È˲ÎÔíÜÕ Rg1¡¢È˲ÎÔíÜÕ Rb1)¡¢220 nm(Îåζ×Óõ¥¼×)¡¢250 nm(Îåζ×Ó´¼¼×)¡¢268 nm(µ¤·ÓËáB)ºÍ 270 nm(µ¤²ÎͪIIA)£¬£¬£¬ £¬£¬Ìå»ýÁ÷Á¿ 1.0 mL/min£¬£¬£¬ £¬£¬ÖùΠ35 ¡æ£¬£¬£¬ £¬£¬½øÑùÁ¿ 10 μL¡£¡£¡£¡£¡£½á¹û ÈýÆßÔíÜÕ R1¡¢Îåζ×Ó´¼¼×¡¢µ¤·ÓËá B¡¢Îåζ×Óõ¥¼×¡¢È˲ÎÔíÜÕ Rg1¡¢È˲ÎÔíÜÕ Rb1¡¢µ¤²Îͪ IIA ¼ì²âÖÊÁ¿Å¨¶ÈµÄÏßÐÔ·¶Î§·Ö±ðΪ 0.015¡«0.150¡¢0.077¡«0.766¡¢0.006¡«0.062¡¢0.009¡«0.092¡¢0.050¡«0.503¡¢0.067¡«0.670¡¢0.011¡«0.108 mg/mL;ƽ¾ù¼ÓÑù»ØÊÕÂÊ·Ö±ðΪ 99.5%¡¢99.8%¡¢99.2%¡¢98.9%¡¢96.9%¡¢98.8%¡¢97.1%¡£¡£¡£¡£¡£½áÂÛ ¸Ã·½·¨¼òµ¥¡¢ÓÐЧ¡¢½á¹û׼ȷ£¬£¬£¬ £¬£¬¿ÉÓÃÓÚÒæ°²ÄþÍèÖÐÉÏÊö 7 ÖÖ»îÐԳɷֵÄͬʱ²â¶¨¡£¡£¡£¡£¡£
¹Ø¼ü´Ê£ºHPLC;²¨³¤Çл»·¨;Òæ°²ÄþÍè;ÈýÆßÔíÜÕ R1;Îåζ×Ó´¼¼×;µ¤·ÓËá B;Îåζ×Óõ¥¼×;È˲ÎÔíÜÕ Rg1;È˲ÎÔíÜÕ Rb1; µ¤²ÎͪIIA
¡¶ÖвÝÒ©¡·ºÓÄÏÖÐÒ½Ò©´óѧÕÅÎÄ×Ú ÕÅ象¶HPLC²¨³¤Çл»·¨Í¬Ê±²â¶¨Òæ°²ÄþÍèÖÐ7ÖÖ»îÐԳɷ֡·
ÍƼöÐÂÎÅ
ÕâÀïÊÇռλÎÄ×Ö
ÕâÀïÊÇռλÎÄ×Ö
ÕâÀïÊÇռλÎÄ×Ö
ÕâÀïÊÇռλÎÄ×Ö
ÕâÀïÊÇռλÎÄ×Ö
ÕâÀïÊÇռλÎÄ×Ö
